Prerequisites
The participants are expected to have a Master’s degree or equivalent. Upon agreement, the course may be taken by master’s students holding a Bachelor degree. Please notice that this is a PhD course and thus the level is very high.
Aims
Advanced methods for protein characterisation and functional genomics/proteomics will be introduced, illustrated, discussed and the partipants will have lab exercises with hands on. Emphasis will be placed on separation technologies and mass spectrometry based methods, preparation of samples and analysis of the obtained results. The goal is to provide the students with the ability to apply the presented methods and strategies to their own research and provide them with a solid background within the field in order to understand and utilize the litterature, with regards to both existing and new methods in mass spectrometry and functional proteomics.
Synopsis
- Principles of mass spectrometry based analytical methods for proteins, peptides and post translational modifications, including Maldi Assisted Laser Desorption/Ionization, electrospray ionization, mass analyzers and hybrid instruments.
- Methods for sample preparation prior to MS analysis.
- Strategies for protein/peptide separation/purification prior to MS analysis, including electrophoretic and chromatographic techniques.
- Strategies for analysis of post translational modifications, with special emphasis on phosphorylation and glycosylation.
- Strategies for quantitative analysis of peptide/proteins, e.g. with respect to research in disease markers.
- Computational data analysis and database searching and other relevant bioinformatic analysis.
Evaluation
Project assigmnent, grade by the danish 7-point scale and external censorship. Exercises in the laboratory will be performed in smaller groups. Based on the knowledge obtained during the lectures, computer exercises and litterature studies, the participants are expected to compose a report concerning the laboratory exercises. The obtained results are analysed and presented. The report is expected to including theory, methods, results, conclusion and perspectives. Reexamination after 4th quarter.
Course type
- Lectures: 25 hours, 10 ECTS.
- Tutorials: 5 hours.
- Lab. work: 30 hours.
Teaching period
2nd quarter.
Remarks
E-Learn is used in this course. This course is taught in English.
Valid from
2008-09-01.
