HUPO Education and Training programs
- Date: June 19-21, 2007.
- Location: University of Southern Denmark (SDU), Odense, Denmark.
- Course organizer: Ole Nørregaard Jensen.
Description
- This hands-on course focuses on bioinformatics and data interpretation with respect to mass spectrometric analysis in the field of proteomics.
- The aim of the course is to introduce different bioinformatics tools useful for interpreting of the information given in the mass spectra especially with respect to MALDI-MS analysis of peptide mixtures for protein identification and with respect to both MALDI and ESI-MS/MS analysis of peptides, including determination of post-translational modifications.
- Researchers with some experience in proteomics and protein mass spectrometry will benefit from this course. Documented work/research experience in protein chemistry and biological mass spectrometry, preferably MALDI MS and/or ESI MS is required. Course participants will be accepted based on their written application and CV.
- The number of participants per course is limited to 16.
Course content: Day 1
- Protein identification by mass spectrometry.
- Overview of mass spectrometry based analytical strategies in protein chemistry and proteomics.
- Description of various types of MS and MS/MS data.
- Overview of bioinformatics tools available for proteomics.
Course content: Day 2
- Protein identification by tandem mass spectrometry.
- Introduction of protein and peptide identification by MS and MS/MS and search engines/parameters.
- Introduction to protein fragmentation, spectra interpretation and de novo sequencing.
Course content: Day 3
- Data interpretation and validation; Annotation of post-translational modifications.
- Introduction to spectrum deconvolution, calibration, databases, error tolerant searches, sequencing of modified peptides (neutral loss, diagnostic ions).
Credits
The course credit will correspond to approximately 24 hours (corresponding to 3 ECTS points). The participants will receive a certificate from the University of Southern Denmark.
Language of teaching
English.
