• Cleaning the Autoflex II ion source - Autoflex II ion source cleaning procedure

  • ClinProTools basic workflow - The software performs statistic analysis of peaks from different spectra. It can also run sophisticated mathematical and bioinformatic algorithms for pattern recognition model generation

  • ESI-Q/TOF - Applied Biosystems QSTAR® XL and Elite mass spectrometers. Guidelines and information on extern calibration, syringe connection, collision energy adjustment, needle washing, LC-ESI interface handling, data acquisition, postprocessing and database search

  • Glycogen content in liver homogenates - Glucose is measured before and after glycogen hydrolysis in a strong acid. The glycogen concentration is obtained by subtracting the free glucose from the glucose released by hydrolysis

  • Hepatic clinical-chemical analysis - Tissue homogenization, lipid extraction, lipid saponification, ADVIA 1650 clinical-chemical system from Siemens, phospholipids, triglycerols, total fatty acids, glucose and glycogen analyses

  • iTRAQ (TM) labeling of proteins - Protein content determination (BCA method), acetone protein extraction, disulfide bridge reduction, trypsination, iTRAQ labeling, column sample loading, freezdrying and sample storage

  • LC-MS/MS systems - Agilent 1100 Module Series® with ChemStation software. Application of three column setups; Strong Cation Exchange (SCX), nano-RP-HPLC-ESI and capillary RP-HPLC-MALDI

  • MALDI-TOF/TOF - Bruker Daltonics Autoflex II mass spectrometer. Guidelines and information on matrix preparation, acquisition modes (linear vs. reflecton mode), LIFT technology, FlexControl automation, FlexAnalysis, BioTools, WARP-LC software, instrument settings and quality control procedures

  • ProteinBrowser results - Compound list information and protein/peptide table parameters. Explanation and interpretation of the output

  • ProteinPilot results - Applied Biosystems/MDS Sciex ProteinPilot (TM) v2.0. Explanation and interpretation of the output

  • Thin layer preparation with HCCA matrix - Thin layer preparation on the AnchorChip™ with 800/384 geometry

  • Tissue, blood and milk sampling - Tissue samples, liver biopsies, blood and milk samples